Physiological proteomics of Bacillus subtilis
Bacillus subtilis is regarded not only as a model organism of Gram positive bacteria but also of functional genomics. Its genome sequence, however, published in 1997 (Kunst et al. 1997) only provides its “blue-print of life”, now functional genomics (transcriptomics, proteomics, metabolomics etc.) is required to bring this genome sequence to cell physiology. The role of proteomics to transfer the “virtual life of the genes to the real life of the proteins” as the main players of life is the main topic of our research program. Proteomes of growing and non-growing cells will provide global information on the regulation of metabolic pathways or on the stress and starvation responses. Stress/starvation proteomic signatures are valuable tools for the prediction of the physiological state of cells or for the prediction of the action mechanisms of unknown drugs.
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